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scoA [2019-08-13 08:59:58]
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scoA [2019-08-13 08:59:58]

succinyl CoA:3-oxoacid CoA-transferase (subunit A)
locus
BSU_38990
pI
6.61
mw
25.25 kDa
protein length
238 aa Sequence Blast
gene length
717 bp Sequence Blast
function
3-hydroxybutyrate utilization
product
succinyl CoA:3-oxoacid CoA-transferase (subunit A)
essential
no
ec
2.8.3.5
synonyms
yxjD
Outlinks
KEGGBsubCycSubtiListUniProtExpression Browser

Genomic Context

      

categories

  • [category|SW 2|Metabolism] → [category|SW 2.4|Lipid metabolism] → [category|SW 2.4.1|Utilization of lipids] → [category|SW 2.4.1.3|Utilization of fatty acids]
  • [category|SW 4|Lifestyles] → [category|SW 4.2|Sporulation] → [category|SW 4.2.1|Sporulation proteins] → [category|SW 4.2.1.4|Sporulation proteins/ other]

    • Gene

    Coordinates
    4,001,976 4,002,692

    The protein

    Catalyzed reaction/ biological activity

  • Succinyl-CoA a 3-oxo acid = succinate a 3-oxoacyl-CoA (according to Swiss-Prot)

    • Protein family

  • 3-oxoacid CoA-transferase subunit A family (with [protein|D8A050BF150C6E5B910C24C0E023F0829EC95BAD|YodS], according to UniProt)

    • Paralogous protein(s)

  • [protein|D8A050BF150C6E5B910C24C0E023F0829EC95BAD|YodS]

    • Structure

  • [PDB|3CDK] (the [protein|A7E61C3D12BBDDD3EFD65B1EDBE280497D7B6CD0|ScoA]-[protein|7F46EDA8F1EB8D9817C62D7D361FF32F9CD814AD|ScoB] complex)

    • Expression and Regulation

    Operons

    genes
    [gene|B416BB368A96926526458B2258BF8AB928D7C15E|hbuT]-[gene|A7E61C3D12BBDDD3EFD65B1EDBE280497D7B6CD0|scoA]-[gene|7F46EDA8F1EB8D9817C62D7D361FF32F9CD814AD|scoB]-[gene|B7B1D28E50244704324920E5A6F110DA8D63F6C0|yxjF]
    description
    [Pubmed|10746760]

    sigma factors

  • [protein|3CB24D493B3282278B7DC61CE3C793DFA815F9E2|SigE]: sigma factor, [Pubmed|12662922], in [regulon|3CB24D493B3282278B7DC61CE3C793DFA815F9E2|SigE regulon]

    • regulatory mechanism

  • [protein|E39AFEB7B7F28969A35C4B51CC6E7819F71C79D9|CcpA]: repression, [Pubmed|12850135,10666464], in [regulon|E39AFEB7B7F28969A35C4B51CC6E7819F71C79D9|CcpA regulon]

    • regulation

  • expressed early during sporulation in the mother cell ([protein|search|SigE]) [Pubmed|12662922]
    • view in new tab

    Biological materials

    Mutant

  • MGNA-B728 (yxjD::erm), available at the [https://shigen.nig.ac.jp/bsub/resource/strainGeneDisrupted/detail/1727 NBRP B. subtilis, Japan]
  • BKE38990 ([gene|A7E61C3D12BBDDD3EFD65B1EDBE280497D7B6CD0|scoA]::erm trpC2) available at [http://www.bgsc.org/getdetail.php?bgscid=BKE38990 BGSC], [Pubmed|28189581], upstream reverse: _UP1_CATATTGATGCCCCTTTCGT, downstream forward: _UP4_GCATCGGGAAAGGGTGAGGC
  • BKK38990 ([gene|A7E61C3D12BBDDD3EFD65B1EDBE280497D7B6CD0|scoA]::kan trpC2) available at [http://www.bgsc.org/getdetail.php?bgscid=BKK38990 BGSC], [Pubmed|28189581], upstream reverse: _UP1_CATATTGATGCCCCTTTCGT, downstream forward: _UP4_GCATCGGGAAAGGGTGAGGC

    • References

  • 17363272,10746760,16672620,12662922,12850135,10666464